primary mouse monoclonal antibodies against cd4 sp35 Search Results


anti cd4  (OriGene)
90
OriGene anti cd4
The number of inflammatory cells, expression of cytokines and transcription factors in normalized relative quantities (NRQ), described as median (IQR) in the tissue of nasal polyps of ECRS and non-ECRS patients, and normal controls
Anti Cd4, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibodies+against+cd4+sp35/pmc04653844-60-21-26?v=OriGene
Average 90 stars, based on 1 article reviews
anti cd4 - by Bioz Stars, 2026-08
90/100 stars
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mouse monoclonal antibody igg anti cd4  (Cell Marque)
86
Cell Marque mouse monoclonal antibody igg anti cd4
The number of inflammatory cells, expression of cytokines and transcription factors in normalized relative quantities (NRQ), described as median (IQR) in the tissue of nasal polyps of ECRS and non-ECRS patients, and normal controls
Mouse Monoclonal Antibody Igg Anti Cd4, supplied by Cell Marque, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibodies+against+cd4+sp35/pm41096755-154-20-27?v=Cell+Marque
Average 86 stars, based on 1 article reviews
mouse monoclonal antibody igg anti cd4 - by Bioz Stars, 2026-08
86/100 stars
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china rabbit antihuman cd4 antibody sp35 maxin  (Proteintech)
96
Proteintech china rabbit antihuman cd4 antibody sp35 maxin
The number of inflammatory cells, expression of cytokines and transcription factors in normalized relative quantities (NRQ), described as median (IQR) in the tissue of nasal polyps of ECRS and non-ECRS patients, and normal controls
China Rabbit Antihuman Cd4 Antibody Sp35 Maxin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibodies+against+cd4+sp35/pm41776455-61-25-24?v=Proteintech
Average 96 stars, based on 1 article reviews
china rabbit antihuman cd4 antibody sp35 maxin - by Bioz Stars, 2026-08
96/100 stars
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anti cd4  (Abcam)
99
Abcam anti cd4
HEV density was a surrogate marker for T-cell inflamed TME. (A) HEV expression (detected by MECA-79 staining) in surgically resected GC tissue was categorized into low and high status based on the median value of HEV density (27.8/cm 2 ). Lymphocytes were stained with anti-CD8 and <t>anti-CD4</t> antibodies for evaluating their relationship with HEV density. Scale bars: 200 µm. (B) Quantification of the MECA-79 area and the CD8 + and CD4 + areas according to the HEV-low and HEV-high groups was performed, and their significant correlations are shown. Values are presented as mean±SEM. (C) The differences in the expression of 730 immune-related genes were evaluated, and genes for immune checkpoints, TME, and chemokines varied with HEV density. (D) Volcano plot summarizing the effects of HEV density on immune-related gene expression. Red line, p<0.05. (E) GO term enrichment analysis revealed 13 statistically significant biological processes controlled by differentially expressed genes among patients with high HEV density. *P<0.05. GC, gastric cancer; GO, gene ontology; HEV, high endothelial venule; IFN, interferon; MHC, major histocompatibility complex; TME, tumor microenvironment; TNF, tumor necrosis factor.
Anti Cd4, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibodies+against+cd4+sp35/pmc08529985-112-19-23?v=Abcam
Average 99 stars, based on 1 article reviews
anti cd4 - by Bioz Stars, 2026-08
99/100 stars
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cd8(c8/144b)  (Biotium)
99
Biotium cd8(c8/144b)
HEV density was a surrogate marker for T-cell inflamed TME. (A) HEV expression (detected by MECA-79 staining) in surgically resected GC tissue was categorized into low and high status based on the median value of HEV density (27.8/cm 2 ). Lymphocytes were stained with anti-CD8 and <t>anti-CD4</t> antibodies for evaluating their relationship with HEV density. Scale bars: 200 µm. (B) Quantification of the MECA-79 area and the CD8 + and CD4 + areas according to the HEV-low and HEV-high groups was performed, and their significant correlations are shown. Values are presented as mean±SEM. (C) The differences in the expression of 730 immune-related genes were evaluated, and genes for immune checkpoints, TME, and chemokines varied with HEV density. (D) Volcano plot summarizing the effects of HEV density on immune-related gene expression. Red line, p<0.05. (E) GO term enrichment analysis revealed 13 statistically significant biological processes controlled by differentially expressed genes among patients with high HEV density. *P<0.05. GC, gastric cancer; GO, gene ontology; HEV, high endothelial venule; IFN, interferon; MHC, major histocompatibility complex; TME, tumor microenvironment; TNF, tumor necrosis factor.
Cd8(c8/144b), supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibodies+against+cd4+sp35/custom%40bnc040749-100%4036206914?v=Biotium
Average 99 stars, based on 1 article reviews
cd8(c8/144b) - by Bioz Stars, 2026-08
99/100 stars
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cd8(c8/468 + c8/144b)  (Biotium)
99
Biotium cd8(c8/468 + c8/144b)
HEV density was a surrogate marker for T-cell inflamed TME. (A) HEV expression (detected by MECA-79 staining) in surgically resected GC tissue was categorized into low and high status based on the median value of HEV density (27.8/cm 2 ). Lymphocytes were stained with anti-CD8 and <t>anti-CD4</t> antibodies for evaluating their relationship with HEV density. Scale bars: 200 µm. (B) Quantification of the MECA-79 area and the CD8 + and CD4 + areas according to the HEV-low and HEV-high groups was performed, and their significant correlations are shown. Values are presented as mean±SEM. (C) The differences in the expression of 730 immune-related genes were evaluated, and genes for immune checkpoints, TME, and chemokines varied with HEV density. (D) Volcano plot summarizing the effects of HEV density on immune-related gene expression. Red line, p<0.05. (E) GO term enrichment analysis revealed 13 statistically significant biological processes controlled by differentially expressed genes among patients with high HEV density. *P<0.05. GC, gastric cancer; GO, gene ontology; HEV, high endothelial venule; IFN, interferon; MHC, major histocompatibility complex; TME, tumor microenvironment; TNF, tumor necrosis factor.
Cd8(c8/468 + C8/144b), supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibodies+against+cd4+sp35/custom%40bnc040750-100%4037428895?v=Biotium
Average 99 stars, based on 1 article reviews
cd8(c8/468 + c8/144b) - by Bioz Stars, 2026-08
99/100 stars
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cd8a antibody  (NSJ Bioreagents)
99
NSJ Bioreagents cd8a antibody
HEV density was a surrogate marker for T-cell inflamed TME. (A) HEV expression (detected by MECA-79 staining) in surgically resected GC tissue was categorized into low and high status based on the median value of HEV density (27.8/cm 2 ). Lymphocytes were stained with anti-CD8 and <t>anti-CD4</t> antibodies for evaluating their relationship with HEV density. Scale bars: 200 µm. (B) Quantification of the MECA-79 area and the CD8 + and CD4 + areas according to the HEV-low and HEV-high groups was performed, and their significant correlations are shown. Values are presented as mean±SEM. (C) The differences in the expression of 730 immune-related genes were evaluated, and genes for immune checkpoints, TME, and chemokines varied with HEV density. (D) Volcano plot summarizing the effects of HEV density on immune-related gene expression. Red line, p<0.05. (E) GO term enrichment analysis revealed 13 statistically significant biological processes controlled by differentially expressed genes among patients with high HEV density. *P<0.05. GC, gastric cancer; GO, gene ontology; HEV, high endothelial venule; IFN, interferon; MHC, major histocompatibility complex; TME, tumor microenvironment; TNF, tumor necrosis factor.
Cd8a Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibodies+against+cd4+sp35/custom%40v2383%4032649874?v=NSJ+Bioreagents
Average 99 stars, based on 1 article reviews
cd8a antibody - by Bioz Stars, 2026-08
99/100 stars
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rabbit anti-human cd4 (sp35)  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH rabbit anti-human cd4 (sp35)
HEV density was a surrogate marker for T-cell inflamed TME. (A) HEV expression (detected by MECA-79 staining) in surgically resected GC tissue was categorized into low and high status based on the median value of HEV density (27.8/cm 2 ). Lymphocytes were stained with anti-CD8 and <t>anti-CD4</t> antibodies for evaluating their relationship with HEV density. Scale bars: 200 µm. (B) Quantification of the MECA-79 area and the CD8 + and CD4 + areas according to the HEV-low and HEV-high groups was performed, and their significant correlations are shown. Values are presented as mean±SEM. (C) The differences in the expression of 730 immune-related genes were evaluated, and genes for immune checkpoints, TME, and chemokines varied with HEV density. (D) Volcano plot summarizing the effects of HEV density on immune-related gene expression. Red line, p<0.05. (E) GO term enrichment analysis revealed 13 statistically significant biological processes controlled by differentially expressed genes among patients with high HEV density. *P<0.05. GC, gastric cancer; GO, gene ontology; HEV, high endothelial venule; IFN, interferon; MHC, major histocompatibility complex; TME, tumor microenvironment; TNF, tumor necrosis factor.
Rabbit Anti Human Cd4 (Sp35), supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibodies+against+cd4+sp35/pm19734217-96-8-12?v=Biozol+Diagnostica+Vertrieb+GmbH
Average 90 stars, based on 1 article reviews
rabbit anti-human cd4 (sp35) - by Bioz Stars, 2026-08
90/100 stars
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helper t lymphocytes  (Spring Bioscience)
86
Spring Bioscience helper t lymphocytes
HEV density was a surrogate marker for T-cell inflamed TME. (A) HEV expression (detected by MECA-79 staining) in surgically resected GC tissue was categorized into low and high status based on the median value of HEV density (27.8/cm 2 ). Lymphocytes were stained with anti-CD8 and <t>anti-CD4</t> antibodies for evaluating their relationship with HEV density. Scale bars: 200 µm. (B) Quantification of the MECA-79 area and the CD8 + and CD4 + areas according to the HEV-low and HEV-high groups was performed, and their significant correlations are shown. Values are presented as mean±SEM. (C) The differences in the expression of 730 immune-related genes were evaluated, and genes for immune checkpoints, TME, and chemokines varied with HEV density. (D) Volcano plot summarizing the effects of HEV density on immune-related gene expression. Red line, p<0.05. (E) GO term enrichment analysis revealed 13 statistically significant biological processes controlled by differentially expressed genes among patients with high HEV density. *P<0.05. GC, gastric cancer; GO, gene ontology; HEV, high endothelial venule; IFN, interferon; MHC, major histocompatibility complex; TME, tumor microenvironment; TNF, tumor necrosis factor.
Helper T Lymphocytes, supplied by Spring Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibodies+against+cd4+sp35/pmc08668236-93-20-29?v=Spring+Bioscience
Average 86 stars, based on 1 article reviews
helper t lymphocytes - by Bioz Stars, 2026-08
86/100 stars
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cd68 antibody  (NSJ Bioreagents)
99
NSJ Bioreagents cd68 antibody
HEV density was a surrogate marker for T-cell inflamed TME. (A) HEV expression (detected by MECA-79 staining) in surgically resected GC tissue was categorized into low and high status based on the median value of HEV density (27.8/cm 2 ). Lymphocytes were stained with anti-CD8 and <t>anti-CD4</t> antibodies for evaluating their relationship with HEV density. Scale bars: 200 µm. (B) Quantification of the MECA-79 area and the CD8 + and CD4 + areas according to the HEV-low and HEV-high groups was performed, and their significant correlations are shown. Values are presented as mean±SEM. (C) The differences in the expression of 730 immune-related genes were evaluated, and genes for immune checkpoints, TME, and chemokines varied with HEV density. (D) Volcano plot summarizing the effects of HEV density on immune-related gene expression. Red line, p<0.05. (E) GO term enrichment analysis revealed 13 statistically significant biological processes controlled by differentially expressed genes among patients with high HEV density. *P<0.05. GC, gastric cancer; GO, gene ontology; HEV, high endothelial venule; IFN, interferon; MHC, major histocompatibility complex; TME, tumor microenvironment; TNF, tumor necrosis factor.
Cd68 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibodies+against+cd4+sp35/custom%40v3175%4026981247?v=NSJ+Bioreagents
Average 99 stars, based on 1 article reviews
cd68 antibody - by Bioz Stars, 2026-08
99/100 stars
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monoclonal rabbit igg anti-cd4 clone sp35  (Bio SB Inc)
90
Bio SB Inc monoclonal rabbit igg anti-cd4 clone sp35
HEV density was a surrogate marker for T-cell inflamed TME. (A) HEV expression (detected by MECA-79 staining) in surgically resected GC tissue was categorized into low and high status based on the median value of HEV density (27.8/cm 2 ). Lymphocytes were stained with anti-CD8 and <t>anti-CD4</t> antibodies for evaluating their relationship with HEV density. Scale bars: 200 µm. (B) Quantification of the MECA-79 area and the CD8 + and CD4 + areas according to the HEV-low and HEV-high groups was performed, and their significant correlations are shown. Values are presented as mean±SEM. (C) The differences in the expression of 730 immune-related genes were evaluated, and genes for immune checkpoints, TME, and chemokines varied with HEV density. (D) Volcano plot summarizing the effects of HEV density on immune-related gene expression. Red line, p<0.05. (E) GO term enrichment analysis revealed 13 statistically significant biological processes controlled by differentially expressed genes among patients with high HEV density. *P<0.05. GC, gastric cancer; GO, gene ontology; HEV, high endothelial venule; IFN, interferon; MHC, major histocompatibility complex; TME, tumor microenvironment; TNF, tumor necrosis factor.
Monoclonal Rabbit Igg Anti Cd4 Clone Sp35, supplied by Bio SB Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibodies+against+cd4+sp35/pmc05833069-126-26-29?v=Bio+SB+Inc
Average 90 stars, based on 1 article reviews
monoclonal rabbit igg anti-cd4 clone sp35 - by Bioz Stars, 2026-08
90/100 stars
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pdcd1 antibody / pd-1 / pd1  (NSJ Bioreagents)
99
NSJ Bioreagents pdcd1 antibody / pd-1 / pd1
HEV density was a surrogate marker for T-cell inflamed TME. (A) HEV expression (detected by MECA-79 staining) in surgically resected GC tissue was categorized into low and high status based on the median value of HEV density (27.8/cm 2 ). Lymphocytes were stained with anti-CD8 and <t>anti-CD4</t> antibodies for evaluating their relationship with HEV density. Scale bars: 200 µm. (B) Quantification of the MECA-79 area and the CD8 + and CD4 + areas according to the HEV-low and HEV-high groups was performed, and their significant correlations are shown. Values are presented as mean±SEM. (C) The differences in the expression of 730 immune-related genes were evaluated, and genes for immune checkpoints, TME, and chemokines varied with HEV density. (D) Volcano plot summarizing the effects of HEV density on immune-related gene expression. Red line, p<0.05. (E) GO term enrichment analysis revealed 13 statistically significant biological processes controlled by differentially expressed genes among patients with high HEV density. *P<0.05. GC, gastric cancer; GO, gene ontology; HEV, high endothelial venule; IFN, interferon; MHC, major histocompatibility complex; TME, tumor microenvironment; TNF, tumor necrosis factor.
Pdcd1 Antibody / Pd 1 / Pd1, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+mouse+monoclonal+antibodies+against+cd4+sp35/custom%40v7999%4032066405?v=NSJ+Bioreagents
Average 99 stars, based on 1 article reviews
pdcd1 antibody / pd-1 / pd1 - by Bioz Stars, 2026-08
99/100 stars
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Image Search Results


The number of inflammatory cells, expression of cytokines and transcription factors in normalized relative quantities (NRQ), described as median (IQR) in the tissue of nasal polyps of ECRS and non-ECRS patients, and normal controls

Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

Article Title: T-cell phenotypes in chronic rhinosinusitis with nasal polyps in Japanese patients

doi: 10.1186/s13223-015-0100-2

Figure Lengend Snippet: The number of inflammatory cells, expression of cytokines and transcription factors in normalized relative quantities (NRQ), described as median (IQR) in the tissue of nasal polyps of ECRS and non-ECRS patients, and normal controls

Article Snippet: The following primary antibodies were used for identifying inflammatory cells in the specimens: anti-CD3 (rabbit monoclonal, clone SP7; Nichirei, Tokyo, Japan), anti-CD4 (rabbit monoclonal, clone SP35; Acris Antibodies Inc, San Diego, California, USA), anti-CD8 (mouse monoclonal, clone C8/144B; Nichirei), and anti-FOXP3 (mouse monoclonal, clone 236A/E7; Abcam, Tokyo, Japan).

Techniques: Expressing

Photomicrographs showing immunohistochemical staining for CD3 (T-cells; a ), FOXP3 ( b ), CD4 ( c ) and CD8 ( d ) in non-ECRS polyps. Scale bar 100 μm

Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

Article Title: T-cell phenotypes in chronic rhinosinusitis with nasal polyps in Japanese patients

doi: 10.1186/s13223-015-0100-2

Figure Lengend Snippet: Photomicrographs showing immunohistochemical staining for CD3 (T-cells; a ), FOXP3 ( b ), CD4 ( c ) and CD8 ( d ) in non-ECRS polyps. Scale bar 100 μm

Article Snippet: The following primary antibodies were used for identifying inflammatory cells in the specimens: anti-CD3 (rabbit monoclonal, clone SP7; Nichirei, Tokyo, Japan), anti-CD4 (rabbit monoclonal, clone SP35; Acris Antibodies Inc, San Diego, California, USA), anti-CD8 (mouse monoclonal, clone C8/144B; Nichirei), and anti-FOXP3 (mouse monoclonal, clone 236A/E7; Abcam, Tokyo, Japan).

Techniques: Immunohistochemical staining, Staining

The number of inflammatory cells in mucosal specimens. The number of CD3 positive cells (T cells) ( a ), CD4 positive cells ( b ) and CD8 positive cells ( c ) per field in the ECRS and non-ECRS polyps, as well as the non-CRS controls. Data in box-and-whisker plots represent the median, lower and upper quartile and the minimum to maximum value. ×, outliers ( † p < 0.005, **p < 0.01, *p < 0.05)

Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

Article Title: T-cell phenotypes in chronic rhinosinusitis with nasal polyps in Japanese patients

doi: 10.1186/s13223-015-0100-2

Figure Lengend Snippet: The number of inflammatory cells in mucosal specimens. The number of CD3 positive cells (T cells) ( a ), CD4 positive cells ( b ) and CD8 positive cells ( c ) per field in the ECRS and non-ECRS polyps, as well as the non-CRS controls. Data in box-and-whisker plots represent the median, lower and upper quartile and the minimum to maximum value. ×, outliers ( † p < 0.005, **p < 0.01, *p < 0.05)

Article Snippet: The following primary antibodies were used for identifying inflammatory cells in the specimens: anti-CD3 (rabbit monoclonal, clone SP7; Nichirei, Tokyo, Japan), anti-CD4 (rabbit monoclonal, clone SP35; Acris Antibodies Inc, San Diego, California, USA), anti-CD8 (mouse monoclonal, clone C8/144B; Nichirei), and anti-FOXP3 (mouse monoclonal, clone 236A/E7; Abcam, Tokyo, Japan).

Techniques: Whisker Assay

HEV density was a surrogate marker for T-cell inflamed TME. (A) HEV expression (detected by MECA-79 staining) in surgically resected GC tissue was categorized into low and high status based on the median value of HEV density (27.8/cm 2 ). Lymphocytes were stained with anti-CD8 and anti-CD4 antibodies for evaluating their relationship with HEV density. Scale bars: 200 µm. (B) Quantification of the MECA-79 area and the CD8 + and CD4 + areas according to the HEV-low and HEV-high groups was performed, and their significant correlations are shown. Values are presented as mean±SEM. (C) The differences in the expression of 730 immune-related genes were evaluated, and genes for immune checkpoints, TME, and chemokines varied with HEV density. (D) Volcano plot summarizing the effects of HEV density on immune-related gene expression. Red line, p<0.05. (E) GO term enrichment analysis revealed 13 statistically significant biological processes controlled by differentially expressed genes among patients with high HEV density. *P<0.05. GC, gastric cancer; GO, gene ontology; HEV, high endothelial venule; IFN, interferon; MHC, major histocompatibility complex; TME, tumor microenvironment; TNF, tumor necrosis factor.

Journal: Journal for Immunotherapy of Cancer

Article Title: High endothelial venule is a surrogate biomarker for T-cell inflamed tumor microenvironment and prognosis in gastric cancer

doi: 10.1136/jitc-2021-003353

Figure Lengend Snippet: HEV density was a surrogate marker for T-cell inflamed TME. (A) HEV expression (detected by MECA-79 staining) in surgically resected GC tissue was categorized into low and high status based on the median value of HEV density (27.8/cm 2 ). Lymphocytes were stained with anti-CD8 and anti-CD4 antibodies for evaluating their relationship with HEV density. Scale bars: 200 µm. (B) Quantification of the MECA-79 area and the CD8 + and CD4 + areas according to the HEV-low and HEV-high groups was performed, and their significant correlations are shown. Values are presented as mean±SEM. (C) The differences in the expression of 730 immune-related genes were evaluated, and genes for immune checkpoints, TME, and chemokines varied with HEV density. (D) Volcano plot summarizing the effects of HEV density on immune-related gene expression. Red line, p<0.05. (E) GO term enrichment analysis revealed 13 statistically significant biological processes controlled by differentially expressed genes among patients with high HEV density. *P<0.05. GC, gastric cancer; GO, gene ontology; HEV, high endothelial venule; IFN, interferon; MHC, major histocompatibility complex; TME, tumor microenvironment; TNF, tumor necrosis factor.

Article Snippet: They were then incubated with anti-peripheral node addressin (PNAd) (rat, clone MECA-79; Santa Cruz), anti-CD8 (rabbit, clone SP57; Ventana), anti-CD4 (rabbit, clone SP35; Abcam), anti-FoxP3 (mouse, clone 236A/E7; Abcam), and anti-PD-L1 (rabbit, clone SP263; Ventana) at 4°C, overnight.

Techniques: Marker, Expressing, Staining

Immune-related gene expression profile explained the anti-tumor immunity of HEV-high GC. (A) HEV-high GC had increased expression of immune cell subsets, including dendritic cells, cytotoxic and helper T cells, and B cells. (B) Fold changes of gene expression of immune cell subsets in HEV-high GC compared to HEV-low GC. Values are presented as mean±SEM. *P<0.05. (C) Gene expression of immune-modulating chemokines and interleukins was higher in HEV-high GC. (D) Fold changes of chemokines and interleukins in HEV-high GC compared with HEV-low GC. Values are presented as mean±SEM. *P<0.05. (E) Comparison of gene expression related to TME, type I or II interferon, Th1 and Th2 response, EC–LC interaction, and inhibitory and agonistic immune checkpoint between HEV-high and HEV-low GC. Values are presented as mean±SEM. *P<0.05. EC, endothelial cell; GC, gastric cancer; HEV, high endothelial venule; IC, immune checkpoint; IFN, interferon; LC, lymphocyte; NK, natural killer; TME, tumor microenvironment.

Journal: Journal for Immunotherapy of Cancer

Article Title: High endothelial venule is a surrogate biomarker for T-cell inflamed tumor microenvironment and prognosis in gastric cancer

doi: 10.1136/jitc-2021-003353

Figure Lengend Snippet: Immune-related gene expression profile explained the anti-tumor immunity of HEV-high GC. (A) HEV-high GC had increased expression of immune cell subsets, including dendritic cells, cytotoxic and helper T cells, and B cells. (B) Fold changes of gene expression of immune cell subsets in HEV-high GC compared to HEV-low GC. Values are presented as mean±SEM. *P<0.05. (C) Gene expression of immune-modulating chemokines and interleukins was higher in HEV-high GC. (D) Fold changes of chemokines and interleukins in HEV-high GC compared with HEV-low GC. Values are presented as mean±SEM. *P<0.05. (E) Comparison of gene expression related to TME, type I or II interferon, Th1 and Th2 response, EC–LC interaction, and inhibitory and agonistic immune checkpoint between HEV-high and HEV-low GC. Values are presented as mean±SEM. *P<0.05. EC, endothelial cell; GC, gastric cancer; HEV, high endothelial venule; IC, immune checkpoint; IFN, interferon; LC, lymphocyte; NK, natural killer; TME, tumor microenvironment.

Article Snippet: They were then incubated with anti-peripheral node addressin (PNAd) (rat, clone MECA-79; Santa Cruz), anti-CD8 (rabbit, clone SP57; Ventana), anti-CD4 (rabbit, clone SP35; Abcam), anti-FoxP3 (mouse, clone 236A/E7; Abcam), and anti-PD-L1 (rabbit, clone SP263; Ventana) at 4°C, overnight.

Techniques: Expressing